腭扁桃体中几种杀伤功能相关分子的表达

Expression and distribution of several cytotoxicity associated molecules in palatine tonsil

目的探讨CD56、PTA1、LA IR-1等与非特异性免疫反应有关的分子在腭扁桃体组织中的表达及分布。方法首先用间接免疫荧光染色流式细胞术分析的方法观察腭扁桃体细胞(PTC)在IL-2活化前后3种分子的表达情况。然后用免疫组化确定CD56表达的部位,以原位杂交法确定PTA1表达的部位。结果 在静止PTC中CD56、PTA1、LA IR-1的表达率分别为3.8%、1.9%和35.1%。经IL-2活化后表达率上升为5.9%,19.2%和54.8%。免疫组化法表明CD56+细胞散在地分布于生发中心。原位杂交法表明,PTA1主要分布于腭扁桃体的滤泡旁区,少量分布在生发中心和内皮细胞上。结论 CD56、PTA1、LA IR-1这3种分子在腭扁桃体细胞中均有表达,且能被IL-2诱导。

Objective To study the expression and distribution of CD56,PTA1 and LAIR-1,which are involved in innate immune diseases,in palatine tonsil cells(PTCs).Methods First,immunostaining and FCM analysis were used to find out the ratio of CD56,PTA1 and LAIR-1 positive cells in PTCs before and after IL-2 stimulation.Then immunohistochemistry and in situ hybridization were used to show where these positive cell distributed.Results By FCM we found out about 3.8%,1.9% and 35.1% of PTCs were CD56,PTA1 and LAIR-1 positive each.After stimulated by IL-2,they rose to 5.9%,19.2% and 54.8%,accordingly.Immunohistochemistry showed CD56 positive cells were located in germinal center.In situ hybridization showed PTA1 positive cells were mostly localized in parafollicules and endothelial cells.Conclusion Conclusion PTC expresses three cytotoxicity associated molecules,i.e.CD56,PTA1 and LAIR-1,which can be upregulated by IL-2.