基于原子力显微镜的微悬臂传感器测定溶菌酶

Determination of Lysozyme Using Microcantilever Sensor Based on Atomic Force Microscopy

提出了一种基于原子力显微镜AFM技术的微悬臂检测溶菌酶的新型传感器技术。通过利用AFM激光束对溶菌酶吸附前后的微悬臂形变程度进行检测,研究了溶菌酶在正十二硫醇修饰微悬臂上的吸附。在最佳条件下,溶菌酶浓度在0.01～100μg/L范围内,其浓度的对数值与微悬臂偏转值呈良好的线性关系,相关系数达到0.998;检出限5.0ng/L。将该法用于药物制剂中溶菌酶含量的测定,获得了满意的结果。

A novel sensor based on an atomic force microscopy(AFM) microcantilever was developed to detect lysozome in a pharmaceutical formulation sample.By measuring the cantilever bending,using the optical reflection technique,the lysozyme adsorption by a dodecanethiol-modified cantilever surface was detected in real time.Under optimal conditions,a linear relationship was established between the deflection of the cantilever and the logarithm of the concentration of lysozome in the concentration range of 0.01-100 μg/L with a correlation coefficient of 0.998;the limit of detection(S/N = 3) was 5.0 ng/L.The method described has been applied to determine lysozyme in pharmaceutical formulation sample with satisfactory results.