丝素蛋白/羟基磷灰石多孔复合材料修复骨缺损研究

Study on Porous Silk Fibroin/ hydroxyapatite Composites Used as Bone Defect Treatment

为了探讨丝素蛋白/羟基磷灰石(SF/HA)复合多孔支架材料的制备及其用于骨缺损的修复,采用超声波凝胶干燥法制备了SF/HA复合材料;以脱胶茧丝为增强材料,水溶性淀粉为制孔剂,通过去离子水萃取法除去淀粉,制备了SF/HA多孔复合材料。对其孔隙率及抗压强度进行测试,并将其植入兔股骨缺损处观察修复的情况。SF/HA多孔复合材料的孔隙率接近75%,孔径尺寸分布约从几微米到400μm,并且孔隙之间相互贯通,其抗压强度可达10 MPa以上,植入兔股骨缺损处未见引起骨组织明显的炎症反应及骨坏死,术后12周发现基本修复骨缺损部位,而空白对照组没有骨生成。SF/HA多孔复合材料可以满足骨组织工程支架的基本要求,用于骨缺损的修复。

To investigate the preparation and the bone defect treatment of the porous silk fibroin/ hydroxyapatite(SF/HA) scaffold composites,the porous composites of SF/HA were prepared by means of dried after sonication-induced gelation.In the process,degummed silk fiber was used as the reinforcement,and watersoluble starch was used as the porogen,which was removed by extraction method with deionized water.The porosity and compressive strength of SF/HA were tested.At the same time,changes of the bone histology were observed by implanting SF/HA into the bone defects of rabbits.The porosity ratio of SF/HA is about 75 % and the compressive strength can exceed 10 MPa.The diameter of the pores is about from several microns to four hundred microns and most of them are circular.The animal experiments show that SF/HA did not result in inflammatory reaction and bone putrescence.The bone defect was repaired by SF/HA composites by 12 weeks after implant operation.Whereas in the control group,by 12 weeks after operation,no callus formed and the defects were not repaired.It is suggested that SF/HA would be a potential scaffold composites for the treatment of bone defects.