康脑液对脑缺血再灌注大鼠脑含水量、脑梗死体积及神经功能的影响

Effects of Kangnaoye on Brain-containing Water Content,Cerebral Infarction Volume and Neurvous Function in Rats with Cerebral Ischemia-reperfusion Injury

目的:观察康脑液对脑缺血再灌注大鼠脑含水量、梗死体积及神经功能评分的影响。方法:线栓法制备大鼠右侧大脑中动脉闭塞(Middle cerebral artery occlusion,MCAO)模型。120只雄性SD大鼠随机分为假手术组、模型组、康脑液大剂量组、康脑液中剂量组、康脑液低剂量组,大鼠缺血2h,分别再灌1d(即24h)、3d、7d,于大鼠苏醒后及再灌24h观察神经功能评分,再灌注24h后测大鼠脑梗死体积,再灌1d、3d、7d分别测各组大鼠脑含水量。结果:与模型组相比,康脑液治疗组大鼠脑梗死体积明显减小、脑含水量明显降低、大鼠神经功能评分显著改善(P<0.05或P<0.01)。结论:康脑液对脑缺血再灌注大鼠神经细胞有保护作用,并能抑制脑水肿形成,减轻脑水肿程度,减轻神经功能损害。

Objective:To investigate the effects of kangnaoye on brain-containing water content,Cerebral Infarction Volume and neurvous function in rats with cerebral ischemia-reperfusion injury.Methods:The model of focal cerebral ischemia and reperfusion was made by thread embolism method.120 healthy male SD rats were randomly divided into 5 groups:sham-operated group,model group,high-dose kangnaoye group(28.6g/kg·d),middle-dose kangnaoye group(14.3g/kg·d)and low-dose kangnaoye group(7.15g/kg·d).The neural function on the rat’s reviving was observed and reobserved 24h after reperfusion.Infarction volume was detected by triphenyltetrazolium chloride(TTC)staining 24h after reperfusion,and brain water content was detected at different time points(1d,3d and 7d after reperfusion).Results:Compared with that of model group,in Kangnaoye treatment groups the cerebral infarct volume decreased obviously,brain moisture content reduced distinctly and the neurobehavioral scores improved visibly(P<0.05 or P<0.01).Conclusion:Kangnaoye can protect the neurocyties of rats with cerebral ischemia-reperfusion injury,reduce cerebral edema and alleviate nerve damage.