高效液相色谱法测定中药延胡索炮制前后延胡索乙素的含量

Determination of Tetrahydropalmatine Content in Corydalis Yanhusuo W.T.Wang before and after Processing by HPLC

目的:测定延胡索中有效成分延胡索乙素的含量。方法:采用美国Agilent ZORBAX SB-C18(4.6×150mm,5μm)色谱柱,流动相甲醇-0.1%磷酸(85:15),检测波长λ=280nm。结果:延胡索乙素在0.02～0.2mg/mL范围内线性关系良好,回收率为100.4%,RSD为1.0%。结论:醋制后延胡索中延胡索乙素的含量明显高于醋制前延胡索中延胡索乙素的含量,测定方法简便、可靠,适合延胡索的质量控制。

Objective:To determine the content of tetrahydropalmatine in Yanhusuo.Methods:The sample was separated on Agilent ZORBAXSB-C18 column(4.6mmx250mm,5μm),with a mobile phase consisting of methyl alcohol-0.1% phosphoric acid(85∶15),a velocity of 1.0mL·min-1,and a detecting wavelength at 280nm.Results:The calibration curve of tetrahydropalmatine was linear between 0.02~0.2mg/mL.The average recovery was 100.4% with RSD1.0%.Conclusion:Assay of Tetrahydropalmatine in Yanhusuo after processing was higher than that before treatment.This method of assaying was proved to be simple and reliable,and can be used for the quality control of Yanhusuo.