YD383、YD439对JAK-STAT6信号传导通路作用的特异性研究

The specifity study of compounds YD383 and YD439 on STAT signal pathway

目的:探讨化合物YD383和YD439对STAT6信号传导通路的作用是否具有特异性。方法:通过瞬时转染的方法将分别依赖于STAT1和STAT6活性的带有荧光素酶报告基因的质粒转染至HeLa细胞株,两种细胞株在各自相应的细胞因子(IFN-γ或IL-4)刺激下激活JAK-STAT1或JAK-STAT6信号传导通路。通过测定荧光素酶的活性来评价细胞株在化合物存在的情况下报告基因的表达。结果:YD383和YD439均能降低相应细胞因子刺激引起的报告基因的表达,并且具有剂量依赖性。随着化合物剂量的增加,报告基因的表达降低(P<0.05)。但化合物引起的两种转染细胞报告基因表达降低的差异比较无统计学意义(P>0.05)。结论:两种化合物对JAK-STAT1和JAK-STAT6信号传导通路均有抑制作用,对JAK-STAT6通路的抑制不具有特异性。

Objective: To study the effects of compounds YD383 and YD439 on STAT6 signal pathway.Methods: HeLa cells were transfected by plasmid with luciferase reporter system.The signal pathway of JAK-STAT1 or JAK-STAT6 was activated by respective cytokines in Hela cells.The effect that compounds YD383 and YD439 on STAT6 signal pathway was evaluated by activity of luciferase measured.Results: Both compounds YD383 and YD439 could decrease expression of gene and in dose-dependent fashion.The genetic expression decreased when dose of compound increased(P<0.05).However,the difference of genic expression was no significance in statistics(P>0.05).Conclusion: Both compounds YD383 and YD439 can suppress the signal pathway of JAK-STAT1 and JAK-STAT6 but no specifity.