小分子硫醇对延边黄牛体细胞核移植重组胚发育的影响
摘要
探讨了在延边黄牛体细胞核移植重组胚早期培养液中添加L-半胱氨酸和β-巯基乙醇(β-ME)两种小分子硫醇类抗氧化剂,对其后续发育的影响,以期筛选出最佳的体外培养条件。结果表明:适合延边黄牛体细胞核移植重组胚后期发育的L-半胱氨酸和β-巯基乙醇的最佳浓度分别为0.6 mmol/L和50μmol/L。
出处
《畜牧与兽医》
北大核心
2010年第6期37-39,共3页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目批准号:30860184
延边大学211工程三期立项项目
参考文献10
-
1林涛,方南洙,宋君博,孙焕林,李钟淑.挤压去核法和盲吸去核法在延边黄牛体细胞核移植中的对比研究[J].江苏农业科学,2009,37(2):178-180. 被引量:5
-
2罗光彬,袁水桥,李东全,文江,王冰松.添加β-巯基乙醇对牛体外受精胚胎体外发育的影响[J].河南农业科学,2006,35(9):131-133. 被引量:5
-
3De Mates DG,Nogucira D,Cortvrindt R,et a1.Capacity of adultand prepubertal mouse oocytes to undergo embryo development in thepresence of cysteamine. Molecular Reproduction and Development . 2003
-
4Gasparrni B,Sayoud H,Neglla G,et a1.GIutathione synthesis dur-ing in vitro maturation of buffalo(Bubalus bubalis)oocytes:effectsof cysteamine on embryo development. The-Riogneology . 2003
-
5Urdaneat A,Jimenez AR,Paramio MT,et al.Cysteamine,glutathi-one and ionomycin treatm ents improve in vitro fertilization of perpu-betal goat oocytes. Zytoze . 2004
-
6Ali AA,Bilodeau JF,Sirard MA.Antioxidant requirements for bo-vine oocytes varies during in vitro maturation,fertilization and devel-opment. Theriogenology . 2003
-
7Lim JM,Liou SS,Hansel W.Intracytoplasmic glutathione concen-tration and the role ofβ-Mercaptoethanol in pre-implantation devel-opmental of bovine embryos. Theriogenoi-ongy . 1996
-
8Takahashi M,Nagai T,Hamano S,et al.Effect of thiolcompounds on in vitro development and intracellularglatathione content of bovine embryos. Biology of Reproduction . 1993
-
9Caamano J N,Ryoo Z Y,Thomas J A,et al.β-Mercaptoethanolenhances blastocyst rate in vitro matured/in vitro fertilized embryos. Biology of Reproduction . 1996
-
10Gardner D K,Lane M.Amino acids and ammonium regulate mouse embryo development in culture. Biology of Reproduction . 1993
二级参考文献20
-
1Wilmut I,Schnieke A E,McWhir J,et al.Viable offspring derived from fetal and adult mammalian cells[J].Nature,1997,385:810-813.
-
2Cibelli J B,Stice S L,Golueke P J,et al.Cloned transgenic calves produced from nonquiescent fetal fibroblasts[J].Science,1998,280:1256-1258.
-
3Polejaeva I A,Chen S H,Vaught T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells[J].Nature,2000,407:86-90.
-
4Lee B C,Kim M K,Jang G,et al.Dogs cloned from adult somatic cells[J].Nature,2005,436:641.
-
5Miyoshi K,Sato K,Yoshida M.In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation[J].Cloning Stem Cells,2006,8:159-165.
-
6Walker S C,Shin T,Zaunbrecher G M,et al.A highly efficient method for porcine cloning by nuclear transfer using in vitromatured oocytes[J].Cloning Stem Cells,2002,4:105-112.
-
7Do minko T,Chan A,Simerly C,et al.Dynamic imaging of the metaphase Ⅱ spindle and maternal chromosomesin bovine oocytes:implications for enucleation efficiency verification,avoidance of parthenogenesis,and successful embryogenesis[J].Biol Reprod,2000,62:150-154.
-
8Greising T,Jonas L.The influence of enucleation on the ultrastructure ofin vitro matured and enucleated cattle oocytes[J].Theriogenology,1999,52:303-312.
-
9Nakayama T,Noda Y,Goto Y,et al.Effects of visible light and other environmental factors on the production of oxygen radicals by hamster embryos[J].Theriogenology,1994,41(2):499-510.
-
10Robl J M,Stice S L.Prospects for the commercial cloning of animals by nuclear transplantation[J].Theriogenology,1989,31:75-84.
共引文献7
-
1袁水桥,罗光彬,李东全,王冰松.牛磺酸在牛胚胎体外发育中的作用研究[J].沈阳农业大学学报,2007,38(4):552-555. 被引量:1
-
2孙新明,张建平,孙弋.山羊体外受精胚胎序贯培养的研究[J].生物技术,2008,18(4):49-51. 被引量:5
-
3翁凡,潘求真.奶牛体细胞克隆胚体外生产的优化研究[J].安徽农学通报,2009,15(17):32-32.
-
4权富生,刘琴,王丽君,马会明,王勇胜,张涌.β-巯基乙醇和发情羊血清对牛颗粒细胞体外培养的影响[J].西北农业学报,2011,20(12):22-25. 被引量:3
-
5尹多,朱玉霞,柳海星,李钟淑,方南洙.自由基吸收类抗氧化剂对延边黄牛体细胞克隆重构胚发育的影响[J].畜牧兽医学报,2012,43(8):1215-1221. 被引量:1
-
6尹多,柳海星,李钟淑,许相勋,方南洙.金属螯合剂对延边黄牛体细胞核移植重组胚发育的影响[J].中国兽医学报,2012,32(10):1566-1568.
-
7王士勇,郑军军,杨月春,刘宗岳,于淼,杨福合.梅花鹿-牛异种体细胞核移植操作方法的研究[J].畜牧兽医学报,2014,45(8):1381-1386.
-
1郭亚钢,王云川.不同割龄的RRIM600和GT1胶乳中硫醇和抗坏血酸的含量[J].云南热作科技,1990,13(3):15-16.
-
2韩岩,尹多,张涛,孙丽丽,李钟淑,方南洙.谷胱甘肽对卵母细胞成熟及胚胎发育影响的研究进展[J].江苏农业科学,2010,38(3):269-270. 被引量:5
-
3刁云飞,李晓霞,李钟淑,方南洙.显微操作液中添加抗氧化剂对延边黄牛体细胞克隆效率的影响[J].畜牧与兽医,2008,40(6):57-59.
-
4胡艳明,祖晶,孙焕林,吴昊,李钟淑,方南洙.FSH和LH对延边黄牛卵母细胞体外成熟及重组胚发育的影响[J].中国畜牧杂志,2009,45(11):18-22. 被引量:4
-
5许丹娜,田加运,何若钢,莫品方,蓝海恩,黄敏瑞,卢晟盛,卢克焕.受精液中添加β-ME、BSA和咖啡因对猪卵母细胞体外受精后早期发育的影响[J].广西农业科学,2008,39(2):226-230. 被引量:8
-
6韩岩,孙丽丽,张宇,李钟淑,方南洙.谷氨酸对延边黄牛体细胞克隆重组胚发育的影响[J].湖北农业科学,2010,49(3):632-634.
-
7尤勇,洪菊生.杉木DNA的简便提取方法[J].林业科学研究,1998,11(1):111-113. 被引量:5
-
8校现周.橡胶胶乳中R—SH的生理作用[J].热带作物研究,1996(3):5-9. 被引量:25
-
9韩岩,魏丽荣,孙丽丽,张涛,李鹏飞,王洪宝,李钟淑,方南洙.甘氨酸对延边黄牛体细胞克隆重组胚发育的影响[J].中国兽医学报,2010,30(3):411-413.
-
10韩岩,姜成国,孙丽丽,王玉涵,朱玉霞,李钟淑,方南洙.精氨酸对延边黄牛体细胞克隆重组胚发育的影响[J].湖北农业科学,2010,49(6):1414-1416.