细丝蛋白A和基质金属蛋白酶9在结直肠腺癌中的表达及意义

Expressions of filamin A and matrix metalloproteinase 9 in colorectal adenocarcinoma and their significance

目的:通过对细丝蛋白A(filamin A,FLNa)和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在结直肠腺癌中表达的研究,探索FLNa和MMP-9与结直肠腺癌发生发展的关系。方法:采用免疫组织化学法、RT-PCR和Western印迹法检测FLNa和MMP-9在60例结直肠腺癌患者癌组织及正常组织标本中的表达情况,并结合临床资料进行分析。结果:FLNa和MMP-9在结直肠腺癌组织中的阳性表达率分别为50.0%和93.3%,而在正常结直肠组织中的阳性率为91.7%和31.7%,差异均有统计学意义(P<0.001)。FLNa和MMP-9在癌组织中的表达呈负相关(r=-0.339,P<0.05)。FLNa和MMP-9的表达与结直肠腺癌的TNM分期、肝转移、淋巴结转移和肠壁浸润深度具有相关性(P<0.05),而与患者的性别、年龄以及肿瘤的部位、大小、分化程度、肉眼形态和组织学类型无关(P>0.05)。RT-PCR和Western印迹法检测结果与免疫组织化学检测结果一致。结论:FLNa和MMP-9的表达水平与结直肠腺癌的发生发展具有高度相关性,有望成为临床诊治的新靶点。

Objective:To study the expressions of filamin A(FLNa) and matrix metalloproteinase-9(MMP-9) in human colorectal adenocarcinoma tissues and explore their correlation with the initiation and progression of colorectal adenocarcinoma.Methods:The expressions of FLNa and MMP-9 in 60 specimens of colorectal adenocarcinoma tissues and normal colorectal tissues were detected using immunohistochemistry technique,reverse transcription polymerase chain reaction(RT-PCR),and Western blot.The correlation between their expression and the clinicopathologic features was analyzed.Results:The positive rates of FLNa and MMP-9 in colorectal adenocarcinoma tissues were 50.0% and 93.3%,respectively,while in normal colorectal tissues they were 91.7% and 31.7%,respectively.The difference was significant(P<0.01).Expression of FLNa was negatively related with expression of MMP-9 in colorectal adenocarcinoma tissues(r=-0.339,P<0.05).Expression levels of FLNa and MMP-9 were correlated with the TNM staging,liver metastasis,lymph node metastasis,and the depth of tumor invasion,but not with the patient's age,gender,lesion site,tumor size,the differentiation degree,gross appearance,and histological classification.The results of RT-PCR and Western blot were consistent with that of immunohistochemistry.Conclusion:The expression levels of FLNa and MMP-9 have a high correlation with the initiation and progression of colorectal adenocarcinoma and have the potential to become a new target for clinical diagnosis and treatment.