摘要
目的:观察选择性环氧化酶2(cyclooxygenase 2,COX-2)抑制剂塞来昔布对人神经胶质瘤细胞株SHG-44的体外抑制增殖和迁移的效应。方法:采用不同浓度塞来昔布(30、50、100和150μmol/L)处理SHG-44细胞24和48 h,并设不用药组作为对照。观察细胞形态学变化,应用MTT法观察塞来昔布对SHG-44细胞的增殖抑制效应,细胞划痕实验观察塞来昔布对SHG-44细胞迁移能力的影响,ELISA检测细胞培养上清液中基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)的含量。结果:塞来昔布对SHG-44细胞的增殖抑制作用呈浓度和时间依赖效应。不同浓度塞来昔布组SHG-44细胞的迁移能力均较对照组下降,且随药物浓度的增加,下降幅度更为明显。ELISA检测发现,不同浓度塞来昔布组细胞培养上清液中MMP-2的含量均较对照组明显下降(P<0.05)。结论:塞来昔布对SHG-44细胞的增殖和迁移均有抑制作用,呈浓度和时间依赖效应。塞来昔布可能通过抑制SHG-44细胞分泌MMP-2,继而抑制细胞的迁移能力。
Objective:To investigate the inhibitory effects of celecoxib,a selective cyclooxygenase 2(COX-2) inhibitor,on the proliferation and migration of human glioblastoma SHG-44 cells in vitro.Methods:The human glioblastoma cell lines SHG-44 were treated with different concentrations of celecoxib(30,50,100,and 150 μmol/L) for 24 and 48 h.The cells without celecoxib treatment were as control group.Cell morphologic changes were observed using optical microscopy.MTT assay was performed to determine the effects of celecoxib on the proliferation of SHG-44 cells.The migration ability of SHG-44 cells was measured using wound healing assay.The content of matrix metalloproteinase 2(MMP-2) in the culture supernatant was detected with ELISA.Results:Celecoxib inhibited the growth of SHG-44 cells in concentration-and time-dependent manners.The migration ability of SHG-44 cells in celecoxib treated group was decreased compared with control group.The decreasing degree was more significant with the increase of the concentration of celecoxib.ELISA found that the level of MMP-2 in the supernatant of celecoxib-treated cells was significantly decreased compared with that of control group(P<0.05).Conclusion:Celecoxib inhibits the proliferation and migration of human glioblastoma cell lines SHG-44 in a concentration-and time-dependent manner in vitro.Celecoxib may inhibit the migration ability of the human glioblastoma cell lines SHG-44 by suppressing MMP-2 secretion.
出处
《肿瘤》
CAS
CSCD
北大核心
2010年第11期913-916,共4页
Tumor
基金
江苏省高校省级重点实验室开放课题(编号:KJS0723)
