bcl-2及cyclinD1 mRNA在膀胱移行细胞癌中的表达

The expression of bcl-2 and cyclinD1 mRNA in bladder transitional cell carcinoma

目的 检测bcl-2及cyclinD1 mRNA在膀胱移行细胞癌中的表达.方法 应用逆转录聚合酶链反应(RT-PCR)的方法检测40例膀胱移行细胞癌组织和10例正常膀胱组织中bcl-2、cyclinD1 mRNA的表达情况.结果 40例膀胱移行细胞癌组织中bcl-2、cyclinD1 mRNA的表达分别为0.650±0.018、0.660±0.022,与其在正常膀胱组织中的表达0.250±0.035、0.320±0.028相比,差异均有统计学意义(P＜0.05).Bcl-2 mRNA在临床分期Tα～T2a中的表达(0.720±0.011)显著高于T2b～T4(0.59±0.017),P＜0.05;cyclinD1 mRNA在Tα～T2a的表达量(0.590±0.021)显著低于T2b～T4(0.750±0.021),P＜0.05.相关分析示相关系数为r=-0.34,P=0.031.结论 bcl-2、cyclinD1 mRNA在膀胱移行细胞癌中过度表达与肿瘤的发生、发展密切相关;可用于膀胱移行细胞癌的诊断和评价预后;在膀胱移行细胞癌的发展及评价预后中,bcl-2、cyclinD1 mRNA呈现出负相关趋势.

Objective To investigate the expression and clinical significance of bcl-2 and cyclinD1 mRNA in bladder transitional cell carcinoma(TCCB). Methods The expression of bcl-2 and cyclinD1 mRNA were detected by RT-PCR in 40 patients with TCCB and 10 normal bladder tissues. Results The expressional level of bcl-2 and cyclinD1 mRNA in 40 TCCB tissues(0.650±0.018,0.660±0.022) were significantly higher than that level in normal tissues(0.250±0.035,0.320±0.028) (P<0.05). The expression of bcl-2 mRNA in the TCCB tissues of clinical stage Tα-T2a was higher than the expression in stage T2b-T4(P<0.05). But cyclinD1 mRNA in the TCCB tissues of clinical stage T2b-T4 was higher than the expression in stage Tα-T2a(P<0.05),and cyclinD1 mRNA and bcl-2 had correlation(P<0.05). Conclusions RT-PCR assay is reliable and sensitive for detecting bcl-2 and cyclinD1 mRNA in carcinoma specimens from TCCB. The high expression of bcl-2 and cyclinD1 mRNA were correlated to the malignancy.