DC-CIK共培养细胞对人结肠癌Caco-2细胞的作用

Effect of DC and CIK co-culture on Caco-2 human colorectal cancer cell lines

目的 观察细胞因子诱导的杀伤细胞(CIK)与同源树突状细胞(DC)共培养后DC-CIK细胞的增殖活性、表型的变化,及其对结肠癌细胞增殖和凋亡的影响.方法 采集健康供者的外周血单个核细胞(MNC),置于37 ℃,5% CO2培养箱培养2 h,收集非贴壁细胞用于诱导培养CIK细胞,贴壁细胞诱导分化出成熟DC,将成熟DC和CIK细胞按1∶4的比例混合培养3 d,用MTT法检测DC-CIK共培养细胞对Caco-2人结肠癌细胞株增殖的影响.结果 DC与CIK细胞共培养后,DC-CIK细胞群的增殖活性和杀伤活性较单纯的CIK细胞更高.结论 DC与CIK共培养细胞是一种增殖活性和细胞毒性均高于CIK细胞的免疫活性细胞.

Objective To investigate the proliferation activities and phenotype changes of DC,CIK and DC-CIK,and their cytotoxicity against colorectal cancer cells in co-culture of DC with CIK.Methods Peripheral blood mononuclear cells (PBMNC) were isolated from healthy adult donors. After incubation of PBMNC for 2 hours,DCs were induced from adherent cells by cytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days,and then were co-cultured with CIK at ratio of 1:4 for 3 days. The cytotoxicity activity against Caco-2 colorectal cancer cell line was detected by MTT assay.Results DC-CIK cells were able to lyse Caco-2 colorectal cancer cells at low effector to target ratios. This effect was significantly enhanced by co-culture with DCs.Conclusions CIK cells have high lytic activity against Caco-2 colorectal cancer cells,which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.