复方肠胃康治疗急性胃肠炎有效部位的研究

Effective Parts of Prescription Changweikang for Treatment of Acute Gastroenteritis

目的筛选复方肠胃康治疗急性胃肠炎的有效部位。方法将复方肠胃康用两种溶剂系统分成9个部位,采用小鼠耳廓肿胀、扭体反应、在体小肠推进和家兔离体肠平滑肌实验方法来观察复方肠胃康不同部位的抗炎、镇痛和解痉作用,筛选有效作用部位。结果部位8的高剂量对二甲苯所致的小鼠耳廓肿胀有显著的抑制作用;部位3和部位9的高剂量对醋酸所致的小鼠扭体反应有显著的镇痛作用;部位1的高剂量具有显著抑制小鼠在体小肠运动的作用;部位2、部位5和部位8对于Ach兴奋家兔离体肠肌均有显著的抑制作用,部位4和部位6对于BaC l2兴奋家兔离体肠肌均有显著的抑制作用。结论部位8是复方肠胃康抗炎的有效部位;部位3和部位9是复方肠胃康镇痛的有效部位;部位1、部位2、部位4、部位5、部位6、部位8是复方肠胃康解痉的有效部位,且解痉的机理有所不同。

Objective To screen effective parts of acute gastroenteritis from extract of Prescription Changweikang.Methods After gaining different parts with different polarity from Changweikang by extracting with two system solvents,ear tumefaction,abdominal constriction,small intestinal vermiculation,isolated ileum tests were adopted to screen these parts according to pharmacodynamics of analgesia,anti-inflammation and spasmolysis.Results High dose of part 8 could obviously inhibit ear tumefaction induced by xylene;high dose of parts 3 and 9 could obviously decrease the number of times of abdominal constriction caused by acetic acid.High dose of part could inhibit small intestinal motility,Parts 2,5 and 8 inhibited constriction of ileum induced by Ach;Parts 4 and 6 inhibited constriction of ileum induced by BaCl2.Conclusion Part 8 is effective solvent part for anti-inflammation;Parts 3 and 9 are effective solvent parts for analgesia;Parts 1,2,4,5,6 and 8 are effective solvent parts for spasmolysis,however,the mechanism for spasmolysis is different.