肾移植术后微嵌合状态的研究

Studies on the microchimerism after kidney transplantation

目的探讨肾移植术后微嵌合体的状态及其与移植肾长期存活的的关系.方法采集以男性为供者的70例女性肾移植患者术后外周血白细胞,用套式PCR扩增单拷贝SRY基因来鉴定患者血中是否存在微嵌合体.结果 70例肾移植术后0.5～10年的女性患者微嵌合体阳性率为58.6%(41/70),2年以内(68%)及5年以上(72%)与2～5年(44.4%)的阳性率比较有明显差异(P<0.05).结论肾移植术后不同时间段嵌合细胞的阳性率有明显差别;长期的微嵌合状态与移植肾长期存活有一定的关系.

Objective To investigate the state of microchimerism after kidney transplantation, and to evaluate the relationship between microchimerism and long term survival of transplanted kidney. Methods Leukocytes were collected from peripheral blood of 70 female recipients having received kidneys from males for the identification of microchimerism by means of amplifying the single copied sex determine region Y gene （SRY） by nested PCR. Results Half to 10 years after renal transplantation, the positive percentage of microchimerism in 70 female patients was 58. 6%（41/70）. These 70 patients were categorized into three groups according to the duration after the transplantation：Group 1 （n= 25）, 0. 5 to 2 years; Group 2 （n=27）, 2 to 5 years; and group 3 （n= 18）, over 5 years. The positive rates of microchimerism for three groups were 68% （17/25）, 44. 4 % （11/27） and 72% （13/18）, respectively. The positive rates in both group 1 and group 3 were significantly different compared with that in group 2 （P〈0.05）. Conclusions After renal transplantation the positive rate of microchimerism varied with the passage of time, and the existence of microchimerism state might benefit the transplanted kidney by prolonging the surrival time of the transplant.