摘要
[目的]采用RAPD法研究孝感荸荠和野生荸荠的遗传差异性。[方法]利用随机扩增多态性DNA(RAPD)技术,对地方栽培种孝感荸荠、野生荸荠、蒲草和扁杆藨草的基因组DNA进行了遗传差异性分析。[结果]共筛选出841、842、807和840 4个随机引物,其中841随机引物的扩增产物多态性明显,获得清晰、重复性好的条带。聚类分析结果显示,栽培荸荠与野生荸荠的亲缘性相对于蒲草与扁杆藨草而言较近。[结论]为培植优质荸荠新品种提供了理论依据。
[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA(RAPD) technology.[Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulate and Scirpus planiculmis Fr.Schmidt were analyzed by RAPD technology.[Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear a...
出处
《安徽农业科学》
CAS
北大核心
2010年第36期20551-20553,共3页
Journal of Anhui Agricultural Sciences
基金
湖北省自然科学基金项目(2005ABA084)
湖北省教育厅重大项目(04Z002)
关键词
荸荠
RAPD
聚类分析
遗传差异性
Water chestnut
RAPD
Cluster analysis
Genetic diversities
