荧光原位杂交与免疫组化检测乳腺癌组织Her-2/neu基因扩增或表达情况的比较

Assessment of Her-2/neu status in breast cancer: a comparison of fluorescence in situ hybridization and immunohistochemistry

目的比较荧光原位杂交法(FISH)与免疫组化法(IHC)对乳腺癌组织中Her-2状态检测结果的差异性和相关性。方法以IHC法检测20例乳腺癌细胞膜上的Her-2蛋白,以FISH法检测上述乳腺癌细胞内的Her-2/neu基因扩增情况,以χ2检验对两种方法检测结果的相关性进行分析。结果两种方法检测结果的总符合率为85·0%,二者之间存在显著正相关(χ2=80·00,P=0·000)。结论IHC法与FISH法在检测乳腺癌Her-2状态方面有良好的一致性。

Objective To compare two assay methods, namely immunohistochemistry （IHC） and fluorescence in situ hybridization （FISH）, in the detection of Her-2/neu status in mammary cancer tissues. Methods In 20 samples IHC technique was applied to detect the Her-2 protein in the cytomembrane of mammary cancer, and FISH was used to assess the amplification of Her-2/neu gene, and SPSS 10. 0 software was employed to analyze the relationship between the two methods. Results The coincident rate of two methods was 85%, therefore there was a significant positive correlation between the two techniques （X^2 =80.00, P=0.00）. Conclusion The two assay methods, IHC and FISH showed a good coincidence in the detection of Her-2/neu status in mammary cancer tissues.