摘要
目的研究盾叶薯蓣水难溶性提取物的化学成分及对人肝癌细胞HepG2生长的抑制作用。方法采用正、反相硅胶柱色谱方法分离纯化水难溶性提取物的单体化合物,并通过波谱学方法鉴定其结构;采用MTT法评价其体外抑制人肝癌细胞的增殖作用。结果采用化学方法分离并鉴定了5个化合物,分别是薯蓣皂苷元(Ⅰ)、延令草皂苷(Ⅱ)、薯蓣皂苷元纤维二糖苷(Ⅲ)、三角叶皂苷(Ⅳ)和盾叶皂苷I(Ⅴ);5个化合物对人肝癌细胞的增值具有明显的抑制作用,其中化合物Ⅴ对人肝癌细胞HepG2的生长抑制作用最强。结论盾叶皂苷Ⅰ具有明显的抗癌活性。
OBJECTIVE To study chemical constituents of the water-insoluble extract from Dioscorea zingiberensis C. H. Wright and cell growth inhibitive effects of chemical constituents against HepG2 cells. METHODS The chemical constituents of waterinsoluble extracts were isolated and purified by silica gel and RP-C18 column chromatography. Their structures were identified by spectral analysis. Anti-proliferative effects of saponins against HepG2 cells were evaluated by MTT assay in vitro. RESULTS Five compounds were i...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第5期527-529,共3页
West China Journal of Pharmaceutical Sciences
基金
国家十一五项目(No2006BAB04A14)
"863"计划专项资助(2007AA021810)
关键词
盾叶薯蓣
化学成分
甾体皂苷
人肝癌细胞HEPG2
盾叶皂苷I
Dioscorea zingiberensis
Chemical constituents
Steroidal saponins
Human hepatocellular carcinoma HepG2 cells
Zingiberensis saponin I