丹参、雪山鼠尾草、云南鼠尾草的质量比较和聚类分析

Comparative study of quality and hierachical cluster analysis of Salvia miltiorrhiza,S. evansiana and S. yunnanensis

目的通过测定比较不同产地丹参药材及其近缘植物雪山鼠尾草和云南鼠尾草中有效成分的含量,建立一种快速鉴别、综合评价正品丹参的方法。方法采用HPLC法测定不同产地的丹参、雪山鼠尾草和云南鼠尾草中丹参酮IIA、丹酚酸B和原儿茶醛的含量,利用雷达图评价指标成分,并根据测得的含量对样品进行聚类分析。结果研究材料中丹参酮IIA和原儿茶醛含量的变化范围较大,丹酚酸B含量相对较稳定;来自四川、河南和山东的丹参综合质量较高;聚类分析将8个样品聚成2组,其中丹参样品聚为一组,云南鼠尾草和雪山鼠尾草聚为另一组。结论所建方法简便、重复性好、结果可靠,是一种评价药材质量、鉴别正品丹参的快速、准确的方法 。

OBJECTIVE To develop a fast method for differention and estimation of the Salvia miltiorrhiza,determine the three components in Salviae miltiorrhizae from various sources,S.evansiana and S.yunnanensis.METHODS The contents of three compounds,tanshinone IIA,salvianolic acid B and protoctechual dehyde I,from Salviae miltiorrhizae in different producing areas,S.evansiana and S.yunnanensis were determined by using HPLC and estimated by radar-gram.Hierarchical clustering analysis was applied to analyze the data.R...