HPLC测定犬血浆中的头孢他美钠

Determination of Cefetamet sodium in dog plasma by HPLC

目的采用HPLC测定犬血浆中的头孢他美钠。方法采用高氯酸直接沉淀血浆蛋白,离心后取上清液进样测定。采用Hypersil BDS C18色谱柱(200 mm×4.6 mm,5μm),流动相为甲醇-5 mmol.L-1高氯酸(24:76,pH2.62),流速1.0 mL.min-1,柱温35℃,检测波长265 nm。结果头孢他美钠0.6032~603.2475μg.mL-1与峰面积的线性关系良好(r=0.9998),低、中、高浓度的方法回收率分别为96.65%、87.41%、95.28%,日内RSD分别为1.25%、7.07%、4.61%(n=5),日间RSD分别为6.33%、10.26%、1.88%(n=5)。结论所建方法简便、灵敏准确,可为头孢他美钠及相关制剂的体内研究提供参考。

OBJECTIVE To establish an HPLC method for the determination of Cefetamet sodium in dog plasma.METHODS After precipitated protein in plasma sample with perchloric acid,the sample was centrifuged for 15 min.The supernatant was determined on a Hypersil BDS C18 column(200 mm×4.6 mm,5 μm).The experiment was performed with mobile phase of methanol-5 mmol·L-1 perchloric acid(24:76,pH2.62)at a flow rate of 1.0 mL·min-1 and detected at 265 nm.RESULTS The linear range was 0.6032-603.2475 μg·mL-1(r=0.9998).The recover...