摘要
目的建立HPLC测定盐酸奥洛他定滴眼液中苯扎氯铵含量的方法。方法以氰基键合硅胶为填充剂;乙腈-水-三乙胺为440∶560∶1,用磷酸调pH 3.4为流动相,流速1.0 mL/min;检测波长210 nm。结果苯扎氯铵在0.05～0.15 mg/mL范围内呈良好的线性关系(r=0.999 8);平均回收率99.7%(n=9,RSD=0.69%)。结论此方法简便灵敏准确,重复性好,可用于测定盐酸奥洛他定滴眼液中苯扎氯铵的含量。
Objective To establish an HPLC method for the determination of benzalkonium chloride in olopatadine hydrochloride eye drops.Methods A CN column was used with the mobile phase of acetonitrile-water-triethylamine(440:560:1).The phosphoric acid was used to adjust pH to 3.4.The flow rate was 1.0 mL/min.The detection wavelength was 210nm.Results The calibration curve was linear in the range of 0.05～0.15 mg/mL for benzalkonium chloride(r = 0.999 8).The average recovery was 99.7 %(n = 9,RSD = 0.69 %) for benzalkon...
出处
《食品与药品》
CAS
2010年第11期425-427,共3页
Food and Drug
