摘要
目的了解铜绿假单胞菌临床分离株的耐药性及耐药机制,指导临床合理选用抗生素。方法应用英国先德微生物分析系统检测药物敏感性,通过改良酶提取物头孢西丁和头孢曲松三维试验检测AmpC酶和ESBLs,K-B纸片扩散法检测金属β内酰胺酶。结果74株临床分离铜绿假单胞菌对亚胺培南的敏感率最高,敏感率达82.4%。对头孢吡肟、阿米卡星、派拉西林、环丙沙星、妥布霉素的敏感性较高。单产ESBLs、同时产ESBLs和AmpC酶、单产AmpC酶和单产金属β内酰胺酶的检出率分别为16.2%,9.4%,5.4%,4.0%。结论临床分离铜绿假单胞菌的耐约现象不容忽视,产生高活性β内酰胺酶是导致其耐药的机制之一,亚胺培南仍是治疗铜绿假单胞菌感染最有效的约物。
Objective To i1nvestigate the antimicrobial.resistance and the mechanismof multidrug resistance in PseudomonasP.aeruginosa) clinical isolates,for guiding the rational use of antibiotics.Methods Antimicrobial susceptibility tests were done by the Sensititre-lactamases and/or extended-Spectrum β-lactamasesESBLs) by modified three-dimensional extract test,while metallo β-lactamasesMBLs) were identified using Kirby-Bauer disk diffusions method.Result The susceptbiliy rate of 74 P.aeruginosa clinical isolates to imipenem was the highest about82.4%,and to cefepime,amikacin、piperacillin Ciprofloxacin tobramicin were higher.ESBLs combined with AmpC β-lactamases,AmpC β-lactamases and MBLs producing strains were detected in 16.2%、9.4%、5.4% and 4.0% of P,aeruginosa respectively.Conclusion The resistant phenomenon of P.aeruginosa clinical isolates shoud not be ignored,production of high active β-lactamases is one of resistant mechanisms in P.aeruginosa,imipenem is still the best choice for treating infection caused by P.aeruginosa.
出处
《医学检验与临床》
2006年第1期46-47,共2页
Medical Laboratory Science and Clinics
