摘要
目的:研究基质金属蛋白酶-2(MMP-2),基质金属蛋白酶-9(MMF-9)及其抑制因子(TIMP-2)在不同形成时期的增生性瘢痕中的基因表达变化。方法:提取16例不同发生时期的增生性瘢痕和8例正常皮肤的总RNA后,分离mRNA,用RT-PCR方法检测MMP-2,MMP-9和TIMP-2基因在不同组织中的表达。结果: MMP-2,MMP-9和TIMP-2基因在正常皮肤和增生性瘢痕中都有表达。在增殖期的瘢痕中,这3种基因转录产物的灰度比值分别为(13．5±4．5),(18．4±4．7),(13．6±2．4),与正常皮肤相比明显升高(P<0．05)。在成熟期的瘢痕中这三种基因表达量恢复到正常皮肤水平。结论:MMP-2,MMP-9和TIMP-2基因表达增强可能是增生性瘢痕形成的机制之一,而MMP-2和MMP-9基因表达降低可能与增生性瘢痕达到相对稳定的成熟状态有关。
Objective:To investigate gene transcription of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in normal skin versus hypertrophic scars. Methods: After total RNA of hypertrophic scars with different periods after wound and normal skin was isolated from 16 specimens of hypertrophic scars and 8 specimens of normal skin and was purified to mRNA, gene expression of MMP-2, MMP-9 and TIMP-2 was examined with reverse transcription-polymerase chain reaction (RT-PCR). Results:Gene expression of MMP-2, MMP-9 and TIMP-2 could all be detected in normal skins and hypertrophic scars. In comparison with normal skin, the gray ratios of transcripts of MMP2, MMP9 and TIMP-2 were apparently enhanced in proliferative scars, and decreased to the levels similar to those of normal skins in mature scars. Conclusion: The increments of gene expression of MMP-2, MMP-9 and TIMP-2 might be involved in the formation of hypertrophic scars, while the decrement of MMP-2 and MMP-9 gene expression might be associated with the maturation of hypertrophic scars.
出处
《感染．炎症．修复》
2006年第1期14-17,共4页
Infection Inflammation Repair
基金
国家自然科学基金重大项目(2005CB522603)
国家自然科学基金(30400172)
