脑卒中患者血管紧张素转化酶基因插入/缺失多态性分析

The analysis of angiotensin converting enzyme gene insertion/deletion polymorphism incerebral stroke

目的探讨血管紧张素转化酶(ACE)基因插入/缺失(I/D)多态性与脑卒中的关系。方法用聚合酶链反应(PCR)方法观察比较131名脑卒中患者及与其年龄相匹配的健康人50名的ACE基因I/D多态性。在实验中插入特异引物进行二次PCR扩增,以避免纯合子DD基因型的误判。结果健康组对照、脑梗死组和脑出血组的基因型分布有差别(χ2=13.87,P=0.008),脑梗死组和脑出血组的DD基因型频率高于健康对照组的相应频率(χ2=9.21,P=0.002;χ2=8.76,P=0.003)。健康对照组、脑梗死组和脑出血组的D等位基因频率有差别(χ2=14.23,P=0.005),脑梗死组和脑出血组的D等位基因频率高于健康对照组的相应频率(χ2=11.17,P=0.001;χ2=10.87,P=0.001)。结论ACE基因具有I/D多态性,脑卒中与DD基因型和D等位基因有一定相关性,是可能引发脑卒中的危险遗传因素。

Objective The aim of this study is investigate the relationship between ACE gene I/D polymorphism and cerebral stroke. Methods 131 patients with cerebral stroke were recruited. The control group includes 50 age-match health cases. The I/D polymorphism of ACE gene was analyzed with PCR amplification. To avoid the mistyping, we used an insertion-specific prime. Results DD genotype shows clear differences among control, infarction and hemorrhage groups （X^2 = 13.87, P = 0. 008 ） o The frequencies of DD genotype in the patients with cerebral stroke was higher than normal controls （X^2 = 9. 21, P = 0. 002;X^2 = 8. 76, P = 0. 003 ）. The D allele also exhibits among control, infarction and hemorrhage groups （X^2 =14. 23 ,P =0. 005） o The frequencies of D allele in the patients with cerebral stroke was higher than normal controls（X^2 = 11.17,P =0. 001 ;X^2 = 10. 87,P = 0. 001 ）. Conclusions There was a positive association between the D allele of the ACE polymorphism and cerebral stroke.