类胰蛋白酶在过敏性疾病诊断中的应用

Evaluation of tryptase measurement in diagnosing allergic diseases

目的评价类胰蛋白酶(tryptase)测定在过敏性疾病诊断中的应用。方法应用Unicap100测定变态反应科门诊不同疾病组35例患者血清类胰蛋白酶水平,以30名健康志愿者作为对照,并与血清总IgE进行相关性分析。结果35例患者中,18例慢性荨麻疹缓解期患者,平均类胰蛋白酶水平(4.74±2.54)μg/L;10例既往有明确过敏性休克病史者(4.86±2.55)μg/L;7例严重全身过敏反应急性发作期(17.4±7.87)μg/L;30名健康志愿者(4.36±1.67)μg/L;严重全身过敏反应急性发作期组明显高于其他各组(P<0.01),余各组之间差异无统计学意义;血清类胰蛋白酶水平与总IgE之间没有明确的相关性。结论血清类胰蛋白酶水平在严重全身过敏反应急性期升高,有辅助诊断的意义,但并非适用于所有过敏性疾病的诊断。

Objective To evaluate the use of tryptase in diagnosing allergic diseases. Methods Serum tryptase was measured by Unicap 100 in 35 patients with different types and states of allergic diseases, and 30 healthy volunteers as controls ; Serum tryptases were compared with IgE level. Results Among the 35 allergic patients, 18 cases were with chronic urticaria（4. 74±2. 54） μg/L; 10 cases had past histories of anaphylaxis shocks（4. 86 ±2. 55 ） μg/L ; 7 cases were in acute phase of anaphylaxis （ 17. 4 ±7. 87 ） μg/L ; 30 healthy volunteers （ 4. 36 ± 1.67 ） μg/L. A statistically significant increase in tryptase concentration was found in patients with acute phase of anaphylaxis compared with other groups. No correlation was found between tryptase concentration and total IgE level. Conclusions Measurement of Serum tryptase level could be an additional tool for diagnosis of anaphylaxis but not chronic urticaria.