不同检测系统和样品稀释液对环孢素检测结果的影响

Influence of testing system and sample diluents on cyclosporine analysis

目的比较TDX系统与AXSYM系统检测环孢素的结果,评估不同样品稀释液对超过线性范围的标本进行稀释后对检测结果的影响。方法在TDX和AXSYM系统上检测100例不同浓度的临床标本,对比检测结果。用全血、蛋白磷酸缓冲液、自制样品稀释液和0点定标液对50例超过AXSYM线性范围的临床标本进行稀释,对比检测结果。结果TDX系统与AXSYM系统检测环孢素的结果相差显著,按不同浓度范围对两系统检测结果求出了换算公式。以全血、蛋白磷酸缓冲液作为样品稀释液和以0点定标液作为稀释液的标本检测结果有统计学意义,自制样品稀释液和0点定标液检测结果之间无统计学意义。结论TDX与AXSYM系统检测环孢素结果间的换算公式有助于临床了解方法改变后检测结果的差异,自制样品稀释液可取代0点定标液用于样品稀释。

Objective To obtain conversion formulae of cyclosporine test results between TDX and AXSYM system and evaluate the influence of different sample diluents in over-range samples on AXSYM system. Methods One hundred samples with different concentration were analyzed by TDX and AXSYM system, respectively. The results were compared. Fifty over-range samples were diluted with prepared diluents such as whole blood, phosphate buffer, serf-prepared diluents and zero point calibrator and then analyzed on AXSYM system. Results There was a significant difference in the results between TDX and AXSYM system. Reasonable formulae were concluded based on different concentration ranges. Results obtained by using whole blood and phosphate buffer as diluents differed from that got by using zero point calibrator. In contrast, there was no significant difference between self-prepared diluents and zero point calibrator. Conclusion The conversion formulae are helpful to evaluate the variation of the results while the analysis system was changed. Serf-prepared diluents could be used to replace zero point calibrator as sample diluents.