L-肉碱对奥硝唑致大鼠精子及生育力损伤的保护作用观察

Protective effect of L-carnitine in the injury induced by ornidazole on the spermatozoon and fertility of male rats

目的探讨L-肉碱(LC)对奥硝唑(ORN)所致大鼠精子及生殖功能损伤的保护作用。方法 40只健康成年雄性SD大鼠随机均分为5组,各8只。A组:予以0.5%羧甲基纤维素钠(溶剂)灌胃、1 ml/d;B组:ORN 400mg/(kg·d)灌胃;C组:ORN 800 mg/(kg·d)灌胃;D组:ORN 400 mg/(kg·d)+LC 100 mg/(kg·d)灌胃;E组:ORN 800 mg/(kg·d))+LC 100 mg/(kg·d)灌胃。均连续灌胃20 d,末次给药24 h后,各组均麻醉后处死大鼠4只,取附睾尾部行精子计数,检测精子活动率及存活率;余大鼠与雌鼠合笼行交配试验。结果与A组相比,B、C组大鼠附睾尾部精子计数、活动率和存活率明显下降(P均<0.05),B组平均胚胎数显著降低(P<0.05),C组无交配雌鼠受孕(P<0.01)。与B组相比,D组大鼠附睾尾部计数、精子活动率和存活率明显增加(P均<0.05),平均胚胎数显著增加(P<0.05),与A组近似(P均>0.05)。E组与C组近似(P均>0.05),与A组各项指标比较,P均<0.01。结论 LC对ORN引起的雄性大鼠精子及生育力损伤具有一定的保护作用。

Objective To investigate the protective effect of L-carnitine in the injuryinduced by ornidazole on the spermatozoon and fertility of male rats.Methods 40 male SD rats(200～230 g) were randomly divided into S groups;group A:0.5%sodium carboxymethyl cellulose given by oral gavage;group B:ORN[400 mg/(kg·d)]given by oral gavage ;group C:ORN[800 mg /(kg·d)]given by oral gavage;group D:ORN[400 mg/(kg·d)]plus LC[100 mg/(kg·d)]given by oral gavage;group E:ORN[800 mg/(kg·d)]plus LC[100 mg/(kg·d)]given by oral gavage.All rats were fed for 20 d continuously.24 h after the last administration,half male rats of every group were put to death after anesthetized. The caudal epididymis were taken to test the sperm count,motility and viability;the others were copulated with female in one cage.Results Compared with group A,the sperm count,motility and viability in caudal epididymis decreased significandy in group B(P0.05 ).Conclusion ORN can affect the quality of sperm in caudal epididymis and fertility in male rats,L-carnitine can play a good protective effect on the injury of sperm and fertility in male rats induced by ORN.