安宫牛黄丸对小鼠肝脏细胞色素P450酶系统亚型RNA表达的影响

Effects of An-Gong-Niu-Huang Wan on the expression of cytochrome P450 isoforms in mouse liver

目的探索安宫牛黄丸对小鼠肝脏细胞色素P450酶系亚型mRNA表达的影响。方法 将健康雄性小鼠随机分为3组:空白对照组、安宫牛黄丸低剂量组(1.5 g/kg)、安宫牛黄丸高剂量组(3.0 g/kg),灌胃给药8d,每天1次,末次给药后1h断头取肝脏。提取、纯化总RNA,采用real-timeRT-PCR方法测定肝脏细胞色素P450酶系部分亚型mRNA的表达。结果安宫牛黄丸高剂量增加Cyp1a1 mRNA的表达;低、高剂量均明显增加Cyp2a4、Cyp3a11、Cyp4a10 mRNA的表达;Cyp7b1 mRNA的表达随给药剂量的增加而减少;而对Cyp2e1、Cyp2j5 mRNA的表达几乎没有影响。结论安宫牛黄丸可诱导小鼠肝脏Cyp1a1、Cyp2a4、Cyp3a11、Cyp4a10 mRNA的表达;抑制Cyp7b1mRNA的表达;但对Cyp2e1、Cyp2j5的表达几乎无影响。

Objective To investigate the effects of An-Gong-Niu-Huang Wan(AGNH) on the expression of hepatic cytochrome P450 isoforms in mouse.Methods Adult male mice were given orally with AGNH(1.5 g/kg-1 and 3.0 g/kg,ig) or saline(control) daily for 8 days.One hour after the last does,mice were decapitated and livers removed.Hepatic total RNA was extracted,purified,and subjected to real-time RT-PCR analysis for expression of cytochrome P450 isoforms.Results Compared to control group,the expression of Cyp1a1 increased in high-dose of AGNH(3.0g/ kg),and Cyp2a4,Cyp3a11,Cyp4a10 increased in both doses of AGNH(1.5 and 3.0 g/kg),but the expression of Cyp7b1 decreased in a dose-dependent manner.The expressions of Cyp2e1 and Cyp2j5 were unchanged in AGNH group.Conclusions AGNH administration for 8 days induced the expressions of Cyp1a1,Cyp2a4,Cyp3a11,Cyp4a10 mRNA in mouse liver,inhibited Cyp7b1 mRNA,but had not effect on the levels of Cyp2e1,Cyp2j5 mRNA.