摘要
目的筛查肥胖人群IRS2G1057D多态性并探讨其意义。方法选取辽宁汉族肥胖者225例,其中2型糖尿病(T2DM)组112例,健康对照组113例。用PCRRFLP方法检测IRS2G1057D多态性,结合T2DM发病机制相关的胰岛素分泌和胰岛素作用简易指标的变化探讨其意义。结果(1)IRS2G1057D变异频率在肥胖总研究人群中为28.7%,T2DM组和对照组分别为33.5%和23.9%(P=0.025)。(2)T2DM组DD基因型频率显著高于对照组,分别为13.4%和5.3%(P=0.041)。Logistic回归分析结果显示:GD和DD基因型的OR值分别为1.246和3.991。(3)在T2DM组,DD基因型的腰臀比(WHR)、HOMAIR及OGTT后2h的血糖、胰岛素和C肽均显著高于同组GG基因型,HOMAβ显著低于同组GG基因型。(4)在肥胖对照组,DD基因型的WHR、2h胰岛素、HOMAIR及HOMAβ也分别高于及低于同组GG基因型(均P<0.05)。(5)T2DM组GD、DD基因型的WHR较对照组均有升高趋势,但差异无统计学意义(P>0.05)。结论肥胖尤其是中心性肥胖的DD基因型携带者T2DM患病风险增大。
To investigate frequency of the G1057D variants of insulin receptor substrate-2 （IRS-2） and the relationship between G1057D polymorphism and type 2 diabetes mellitus （T2DM） in Chinese Han obese subjects in Liaoning. Methods 225 Chinese Han obese subjects, including 112 patients with T2DM and 113 normal controls, were selected in Liaoning area. The G1057D polymorphism of IRS-2 gene were detected by PCR-RFLP. Results （1） The frequency of allele D of G1057D variant was 28.7% in all obese subjects, with 33.5% in T2DM group and 23.9% in normal group （P=0. 025）. （2） The frequency of DD genotype was significantly higher in T2DM group than that in normal group （13.4% vs 5.3%, P = 0. 041）. Logistic regression analysis of diabetic risk showed that the OR values for GD and DD genotype of IRS-2 were 1. 246 （P=0. 443） and 0. 399 （P=0. 011）, respectively. （3） WHR, HOMA-IR, 2 hPG, 2 hIns, and 2 hCP were higher in patients with DD genotype than those with GG genotype in T2DM group. However, HOMA-β was lower in DD genotype than in GG genotype in T2DM group （P＜0. 005）. （4） The DD genetype had higher levels of WHR, 2 hIns and HOMA IR, and lower HOMA-β than GG genetype （all P＜0.05） in obese control group. （5）WHR of GD and DD genotypes in obese DM group were higher than those in control group （P＞0.05）. Conclusion The obese subjects with DD genotype for G1057D of IRS-2 may increase the risk for T2DM.
基金
辽宁省科技厅重点科技攻关基金资助项目(200122500116)
