雌激素受体β在大鼠下颌下腺的免疫组织化学定位

Immunohistochemical localization of estrogen receptor p in rat submandibular gland

目的:探讨雌激素β型受体(estrogen receptorβ,ER-β)在大鼠下颌下腺的定位和分布,为进一步研究ER-β在下颌下腺的可能功能提供形态学依据。方法:免疫组织化学SABC法。结果:大鼠下颌下腺浆液性腺细胞、泡心细胞、闰管、颗粒曲管及大于颗粒曲管的各级导管上皮细胞均呈ER-β免疫反应阳性,阳性反应物质主要位于阳性细胞胞质内,核呈阴性反应。结论:大鼠下颌下腺存在ER-β,雌激素可能通过ER-β的介导对下颌下腺功能进行调控。

To observe the localization and distribution of estrogen receptor β （ER-β） in rat submandibular gland, providing morphological evidence for further functional studies of ER-β in submandibular gland. Methods： Immunohistochemical SABC method was used in the experiment. Results： The serous gland cells,centroacinar cells, intercalated duct epithelial cells, granular convoluted epithelial cells and other duct epithelial cells all showed positive immunoreactivity of ER-β. Positive substance was mainly distributed in the cytoplasm and was negative in the nuclei. Conclusion： The rat submandibular gland can synthesize ER-β. The function of rat submandibular gland might be regulated by estrogen through ER-β.