胎儿小肠生长抑素、胃泌素及血管活性肠肽免疫反应细胞的个体发生

Ontogeny of somatostatin,gastrin and vasoactive intestinal peptide inununoreactive cells in fetal small intestine

目的:探讨生长抑素(SS)、胃泌素((Gas)、血管活性肠肽(VIP)免疫反应(IR)细胞在胎儿小肠中的个体发生及其分布。方法:免疫组织化学方法和图像分析技术。结果:SS-IR细胞和(Gas-IR细胞于11周即可见于小肠三段绒毛上皮和尚未分化完全的肠腺细胞间。随着胎龄增长,SS-IR细胞数量和细胞内SS由少至多,其中以十二指肠细胞最多,回肠最少。Gas-IR细胞和细胞内的Gas在十二指肠中随胎龄增长逐渐增多;21周后空肠中则有所减少,回肠中则消失。VIP-IR细胞数量少,整个胎期细胞数量、形态及免疫染色强度均未见明显变化。结论:SS、Gas和VIP在胎儿小肠的内分泌细胞中表达,提示内分泌激素在胎儿小肠的发育过程中起调节作用。

To investigate the ontogeny and distribution of the somatostatin （SS）, gastrin （GAS） and vasoactive intestinal peptide （VIP）-immunoreactive cells in fetal small intestine. Methods： The immunohistoehemical method and image analysis technique were used. Results： SS-IR cells and Gas-IR cells were detected in the epithelium of intestinal villi and undifferentiated glands at the 11st week. The number of SS-IR cells and increased gradually with the growth of fetus, with the highest in the duodenum and the lowest in the ileum. The somatostatin expressed in the SS-IR cells and increased gradually. The number of Gas-IR cells increased in the duodenum with age and decreased in the jejunum, and disappeared in the ileum after 21 weeks. The gastrin expression increased in the Gas-IR cells in the duodenum and decreased in the jejunum and ileum. In the whole fetal period, VIP-IR cells were few and scattered; no variations could be observed during the period. Conclusion： SS, Gas and VIP are expressed in endocrine cells of fetal small intestine and they may regulate the development of fetal small intestine.