山柰苷的人肠内细菌生物转化研究

The biotransformation of kaempferitrin by human intestinal flora

目的探讨人肠内细菌对中药罗汉果中山柰苷的生物转化。方法采用人肠内细菌与山柰苷共温孵培养的方法,通过色谱技术分离、纯化转化产物,应用谱学技术确定转化产物的结构。结果人肠内细菌转化山柰苷产生山柰酚3OαL吡喃鼠李糖苷(阿福豆苷)、山柰酚7OαL吡喃鼠李糖苷、山柰酚和对羟基苯甲酸。结论山柰苷可被人肠内细菌进行生物转化。

Aim To study the biotransformation of kaempferitrin, a major chemical principle of the fruits of Siraitia grosvenori （Swingle） C. Jeffery, with human intestinal flora. Methods The kaempferitrin was incubated with human intestinal flora. The biotransformation products were isolated and purified by chromatographic methods and the structures were determined by spectroscopic techniques. Results Kaempferitrin was converted into kaempferol 3-O-α-L-rhamnoside （afzelin, Ⅰ）, kaempferol 7-O-α-L-rhamnoside （Ⅱ）, kaempferol （Ⅲ） and p-hydroxybenzoic acid （Ⅳ） by human intestinal flora. Conclusion The structure of kaempferitrin can be biotransformatedly converted by human intestinal flora.