低分子质量肝素对糖尿病肾病大鼠肾血管内皮生长因子表达的影响

Effects of low molecular weight heparin on vascular endothelial growth factor expression of early diabetic nephropathy

目的:探讨低分子量肝素(lowmolecularweightheparin,LMWH)对早期糖尿病肾病大鼠的保护作用及其机制。方法:将雄性SD大鼠随机分为正常对照组、糖尿病肾病组和糖尿病肾病LMWH治疗组。分别于1,2,4,6,8周留取肾组织,采用免疫组织化学方法检测肾小球血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)的表达,并进行定量病理分析。结果:正常大鼠肾小球脏层上皮细胞和肾小管上皮细胞可见VEGF表达,糖尿病肾病大鼠除上述细胞外,还可见肾小球系膜细胞、内皮细胞及基底膜VEGF表达。第2周后糖尿病肾病组VEGF阳性肾小球平均光密度、积分光密度均明显高于正常对照组;LMWH治疗4周后,平均光密度及积分光密度与非治疗组相比均明显下降。结论:LMWH可抑制糖尿病肾病大鼠肾VEGF的表达,这可能是其发挥肾保护作用的机制之一。

Objective ： To investigate the effects of low molecular weight heparin （LMWH） on vascular endothelial growth factor（VEGF） expression of early diabetic nephropathy. Methods： Ninety-five male SD rats were randomly divided into three groups： normal control rats, STZ-induced diabetic rats and diabetic rats treated with LMWH. The renal tissues were subjected to immunohistochemical staining after 1, 2,4,6, and 8 weeks＇ treatment respectively to quantify the VEGF expression. Results： Immunohistochemical staining demonstrated an increasing in VEGF positive cells in diabetic rats. It was found that there were significant differences in VEGF staining intensity between diabetic rats and normal control rats and between LMWH treated rats and untreated diabetic rats after two weeks treatment. Conclusion： The inhibition of VEGF expression may be one of the mechanisms of LMWH＇ s renal protective effects on early diabetic nephropathy.