PDCD5和p53在口腔白斑和口腔鳞癌中表达的相关性

Expressions of PDCD5 and p53 in oral leukoplakia and oral squamous cell carcinoma

目的:研究凋亡相关新基因PDCD5与p53在口腔正常黏膜、口腔白斑和口腔鳞癌中表达的相关性及其意义。方法:采用免疫组织化学方法检测17例正常口腔黏膜,60例口腔白斑,30例口腔鳞癌组织标本中PDCD5基因及p53的表达。结果:正常口腔黏膜组PDCD5蛋白染色阳性率为88.2%,白斑组PDCD5蛋白染色阳性率为63.3%,口腔鳞癌组PDCD5蛋白染色阳性率为30%;正常口腔黏膜组P53染色阳性率为0,白斑组P53染色阳性率为31.7%,口腔鳞癌组P53染色阳性率为60%。将PDCD5蛋白和P53在不同病例组织中的染色强度指数(stainingintensityindex,SII)做进一步的Pearson直线相关性分析,提示在口腔正常黏膜、口腔白斑以及口腔鳞癌组织中,PD-CD5SII和P53SII呈明显负相关(r=-0.892,p=0.000)。结论:在口腔白斑、口腔鳞癌组织中,不论PDCD5或p53基因是单独失活,还是同时失活,都可导致口腔黏膜上皮向癌变发展,甚至形成肿瘤;二者同时失活有叠加效应。这两项指标可作为辅助检测口腔黏膜癌变的基因标志物。

Objective： To study the relationship of the expressions of new apoptosis-related gene PDCD5 and p53 in oral normal mucosa, oral leukoplakia and oral squamous cell carcinoma. Methods： The expressions of PDCD5 and p53 were observed separately in 17 samples of oral normal mucosa, 60 of oral leukoplakia, and 30 of oral squamous cell carcinoma by Immunohistochemical means. Results： PDCD5 positive rate in oral normal mucosa was 88.2% , in oral leukoplakia was 63.3% , and in oral squamous cell carcinoma was 30%. P53 positive rate in oral normal mucosa was 0, in oral leukoplakia was 31.7%, and in oral squamous cell carcinoma was 60%. There was a negative relationship between PDCD5 SII and P53 SII in every lesion. Conclusion： It suggests that both PDCD5 and p53 could be used as molecular markers of carcinogenesis for oral epithelium.