静止压力诱导血管平滑肌细胞的增殖与凋亡

Static pressure induces vascular smooth muscle cells proliferation and apoptosis

目的探讨静止压力对血管平滑肌细胞增殖与凋亡的影响。方法将胚胎鼠血管平滑肌细胞系A10细胞分别在正常大气压下、80、100、200mmHg等静止压力下培养;利用MTT法测定细胞成活率,流式细胞仪测试样品的细胞周期与凋亡率。结果在较低压力下(100mmHg)A10细胞成活率增加,细胞增殖,处于G0/G1期的A10细胞百分比显著降低,增殖指数(PI)显著升高,凋亡率与正常无差异;在200mmHg的压力下成活率降低,G0/G1期A10细胞百分比显著升高,细胞增殖受抑制,凋亡率亦显著增加。结论较低的压力可促进血管平滑肌细胞增殖,而过高的压力则可诱导细胞凋亡。

Objective To study the role of static pressure on proliferation and apoptosis ot the vascular smooth muscle cells. Methods Vascular smooth muscle cell line A10 were cultured at normal atmosphere, 80 mmHg, 100 mmHg, 200 mmHg static pressure respectively. Cell viability was determined by MTT assay, cell cycle and apoptosis rate were analyzed by flow cytometer. Results After exposure to lower static pressure （100mmHg） for 48 hour, cell viability and proliferation index （PI） of A10 cell significantly increased, the percentage of A10 cell in G0/G1 phase decreased significantly and the apoptosis rate showed no difference as compared with those exposed to atmospheric pressures. However after exposure of A10 cell to higher static pressure （200 mmHg） for 48 hours, cell viability and proliferation index （PI） significantly decreased, the percentage of A10 cell in G0/G1 phase increased significantly, apoptosis rate significantly increased, as compared with cells exposed to atmospheric pressure. Conclusion Lower static pressure can facilitate vascular smooth muscle cells proliferation, while higher pressure can induce cell apoptosis.