邻苯二甲酸酯类对大鼠睾丸支持细胞毒性作用

Toxicity of phthalates on Sertoli cells of rat testis

目的探讨大鼠睾丸支持细胞间紧密连接结构能否作为邻苯二甲酸酯类(PAE)毒性作用效应的标志物。方法建立大鼠睾丸支持细胞原代双室培养模型,通过光镜、跨上皮电阻测定及免疫荧光定位方法研究PAE对大鼠支持细胞及支持细胞间紧密连接结构的影响。结果PAE染毒后,支持细胞单层破坏、细胞间的嵴线消失、跨上皮电阻值下降、紧密连接相关蛋白ZO-1、F-肌动蛋白和闭锁蛋白表达降低;600μmol·L-1浓度下,单乙基己基邻苯二甲酸酯(MEHP)对睾丸支持细胞的毒性大于单丁基邻苯二甲酸酯(MBP)。结论MEHP和MBP可破坏睾丸支持细胞间的紧密连接结构,跨上皮电阻值可作为PAE睾丸毒作用敏感的效应标志物。

AIM To study whether the tight junctions of Sertoli cells may serve as a biomarker of phthalate effects. METHODS Based on the two-compartment primary Sertoli cells culture model, the effects of phthalates on the tight junctions of Sertoli cells were studied by the methods of light microscope, transmission electron microscope, transepithelial electrical resistance assay and immunofluorescence localization. RESULTS After being treated with phthalates, the damage of Sertoli cells included the destroyed cell monolayer, the loss of ridge line, the decline of transepithelial electrical resistance and the decreased expression of tight junction related proteins, such as ZO-1, F-actin and occludin The toxicity of nlono-2-ethylhexyl phthalate on Senoli cell ,was higher than that of mono-n-butyl phthalate at the same dose. CONCLUSION Phthalates can destroy the tight junctions of Sertoli cells. Transepithelial electrical resistance can be a sensitive bionlarker of phthalate effects on rat testis.