文心兰原球茎增殖培养的研究

The Study on Enrichment Culture of Protocorm in Oncidium

在MS、1/2MS、KC和VW 4种基本培养基中分别添加0.5～5 mg/LBA和1 mg/L NAA的对研究不同培养基对文心兰(Oncidium)原球茎增殖的影响.结果表明,KC的效果最好,VW的效果次之,MS的效果最差.在KC基本培养基中,以KC为培养基,添加3 mg/L BA、0.3 mg/L NAA最适合文心兰原球茎的增殖.在添加3 mg/L BA和0.3 mg/LNAA的KC培养基中,添加有机物促进原球茎增殖,添加椰汁和番茄汁,原球茎增殖差异不显著.

The influence of four minimal medium on enrichment of protocorm is studied.The four minimal medium is MS,1/2MS,KC and VW with 0.5～5.0 mg/L BA and 1 mg/L NAA add separately.The results show that the effect of KC is best,minor VW,the worst MS.The best medium fit for enrichment of protocorm is KC with 3 mg/L BA,0.3 mg/L NAA added.It is marked about protocorm enrichment when added organics in the best medium above;but not marked when added coconut juice and tomato juice to it.