灌注式RCCS生物反应器CFD模拟分析微载体旋转对细胞氧供的影响

Stimulation Analysis of Perfusion Rotary Cell Culture System Bioreactors with Computational Fluid Dynamic Software

目的:探讨利用微重力旋转细胞培养系统(RCCS)生物反应器仿真模拟分析微载体旋转对细胞氧供的影响。方法:联立不可压缩牛顿流体连续性方程和动量守恒方程、微载体内以及流动空间中氧的传递和反应方程,设置参数并求解;以Gamb it建立RCCS网格模型,并用F luent软件在欧拉多相模式下进行生物反应器数值模拟及流体力学分析。结果:以微载体直径及RCCS旋转速度作为自变量,基于欧拉-欧拉多相模型和氧运输公式成功建立RCCS数值分析模型。微载体直径和旋转速度均影响细胞培养效率,直径200、300、400μm的微载体旋转速度分别为10、12、14 r/m in;旋转1 h后直径600μm的微载体RCCS中间区平均氧浓度增加85%。结论:CFD可以定量模拟分析影响微载体及氧供的相关因素,为优化培养条件提供参数。

Objective To simulate the rotary cell culture system(RCCS) liver bioreactor model with computational fluid dynamic(CFD) software and to analyze the characteristic of nutrition supply.Methods A numerical analysis of the RWV bioreactor was conducted by incorporating the Eulerian-Eulerian multiphase and oxygen transport equations.The bead type,size and rotating speed were the control variables in the calculations.Results The present results showed that the rotating speed for appropriate suspensions should to be increased as the size of the bead/cell increasing:10 r/min for 200 μm;12 r/min for 300 μm.As the rotating speed and the bead size increasing from 10 r/min(200 μm) to 18 r/min/(600 μm),the mean oxygen concentration in the 80% midzone of the vessel was increased to 85% after 1 hour rotation due to the high convective flow for 18 r/min(600 μm) case as compared to 10 r/min(200 μm) case.Conclusion The present results may serve as criteria to set the operating parameters for a RWV bioreactor,it might provide a design parameter for an advanced suspension bioreactor for 3D engineered cell and tissue culture.