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PEP-1介导的血红素氧合酶HO-1对缺血再灌注损伤大鼠肝脏TNF-α mRNA表达的影响

Effects of PEP-1 Mediated Heme Oxygenase-1 on TNF-α mRNA Expression in Liver of Rats with Ischemia-reperfusion Injury
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摘要 目的:探讨PEP-1介导的血红素氧合酶-1(HO-1)对缺血再灌注损伤(IR I)大鼠肝脏肿瘤坏死因子(TNF-α)mRNA的影响。方法:SD大鼠经尾静脉注射HO-1(HO-1组)或PEP-1-HO-1(PEP-1-HO-1组)300μg后,制备大鼠肝脏缺血再灌注损伤模型,1、6、12、24 h后用RT-PCR法检测肝组织中TNF-αmRNA水平,另设对照。结果:PEP-1-HO-1组肝脏TNF-αmRNA水平均较单纯IR I组和HO-1组低(P<0.05),且随IR I时间延长而逐渐降低(P<0.05)。结论:PEP-1介导的HO-1融合蛋白降低肝脏TNF-αmRNA表达,可能减轻肝缺血再灌注损伤程度。 Objective To investigate the effects of PEP-1 mediated heme oxygenase-1(PEP-1-HO-1)fusion protein on TNF-α mRNA expression in liver of rats with ischemia-reperfusion injury.Methods SD rats were respectively injected HO-1(HO-1 group) or PEP-1-HO-1(PEP-1-HO-1 group) with dose of 300 μg in tail vein,then the liver ischemia-reperfusion injury model were prepared in these rats,and control group was designed.The TNF-mRNA expression levels in liver were detected with RT-PCR at 1,6,12,24 h,respectively.Results The TNF-α mRNA expression in PEP-1-HO-1 group were significantly lower than those of HO-1 group and IRI group(P<0.05),and the expression was decreased following the injury prolong(P<0.05).Conclusion PEP-1-HO-1 fusion protein could decrease the expression of TNF-α mRNA,so as to lighten liver ischemia-reperfusion injury.
作者 涂华华 王卫星 陈先祥 蔡庆和 何志军 张林菲
机构地区 武汉大学人民医院普外科 湖北医药学院附属人民医院肝胆外科
出处 《湖北医药学院学报》 CAS 2011年第4期364-366,共3页 Journal of Hubei University of Medicine
关键词 PEP-1介导的血红素氧合酶融合蛋白(PEP-1-HO-1) 缺血再灌注损伤 肿瘤坏死因子 PEP-1 mediated heme oxygenase-1 fusion protein Ischemia-reperfusion injury TNF-α
分类号 R363 [医药卫生—病理学]
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参考文献8

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湖北医药学院学报
2011年 第4期

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