生长分化因子5对血管内皮细胞增殖和运动的影响

Effects of growth differentiation factor 5 on proliferation and movement of endothelial cells

目的:探讨生长分化因子5(GDF5)对心肌梗死后血管修复的作用,阐明其修复的机制。方法:体外培养血管内皮细胞,分别以不同浓度(10、50及100μg·L-1)的GDF5作用于细胞,同时设正常对照组,CCK8法和立体培养法检测血管内皮细胞的增殖抑制率和细胞迁移。结果:CCK8实验法,GDF5对血管内皮细胞的增殖有抑制作用,随着GDF5剂量的增加,抑制率也相应地增加达2倍以上,组间比较差异有统计学意义(P<0.05)。立体培养法检测,GDF5对血管内皮细胞的运动有明显促进作用,随着GDF5剂量的增加,细胞运动明显增快,不同浓度GDF5组与对照组比较差异均有统计学意义(P<0.05)。结论:GDF5对血管内皮细胞增殖起到抑制作用,对血管内皮细胞的迁移起到促进作用。

Objective To investigate the effects of growth differentiation factor 5(GDF5) on the proliferation and migration of endothelial cells,and clarify the mechanism of its repair.Methods Vascular endothelial cells were cultivated with different concentrations(10,50,100 μg·L-1) of GDF5,and normal control group was set up at the same time.The cell proliferation was detected by method of CCK8 method.Three-dimensional cultivation was used to investigate the effect of GDF5 on the migration of endothelial cells.Results CCK8 experiment showed that GDF5 inhibited the proliferation of vascular endothelial cells;with the increasing of GDF5 dose,the inhibitory rate was increased correspondingly even more than twice,there were significant differences between various groups(P<0.05).Three-dimensional experiment showed that GDF5 obviously promoted the movement of vascular endothelial cells,with the increasing of GDF5 dose,the cell movement was significantly increased,there were significant differences between GDF5 groups and control group(P<0.05).Conclusion GDF5 can inhibit the proliferation of endothelial cells and promote the migration of endothelial cells.