冻干前后红细胞血型及生物活性指标的检测

Examination of the Blood Groups and Biologal Activity of the Freeze-drying Red Blood Cells

目的检测冻干前、后红细胞血型抗原及其生物学活性,了解冻干前、后红细胞血型抗原及其生物学活性的变化情况。方法红细胞血型抗原的检测采用血型血清学方法,2,3-DPG和ATP用ELISA法。结果在ABO、Rh、MNSs、Kell、Duffy、P血型抗原中,冷冻前、冻干后红细胞血型抗原一致;在Lewis血型抗原中,冷冻前、冻干后红细胞血型抗原有变化。冷冻前、冻干后红细胞2,3-DPG和ATP含量均无明显变化。结论冷冻前、冻干后红细胞Lewis血型系统中的Lea和Leb血型抗原变化有明显差异;冷冻前、冻干后红细胞2,3-DPG和ATP含量变化无统计学意义。

Objective To detect the blood group antigens,the levels of 2,3-DPG and ATP of red blood cells(RBCs) before and after freeze-drying.Also the changes of blood group antigens and biological activity of RBCs before and after RBCs freeze-drying was observed.Methods The blood group antigens of RBCs were detected by the blood group serology,the levels of 2,3-DPG and ATP were detected by ELISA.Results The blood group antigens of RBCs were the same as before and after freeze-drying about ABO,Rh,MNSs,Kell,Duffy and P blood group systems.But the blood group antigens of RBCs of Lewis blood group systems were different before and after freeze-drying.The levels of 2,3-DPG and ATP of RBCs before and after freeze-drying didn′t change significantly.Conclusion The Lea and Leb blood group antigens of RBCs before and after freeze-drying of Lewis blood group systems have changed significantly.The levels of 2,3-DPG and ATP of RBCs before and after freeze-drying havn′t chenged significantly.