不同小鼠IgG对丙型肝炎病毒核心抗原酶联免疫法检测的影响

Effect of Various Mouse-IgG on HCV-cAg ELISA

目的获得最适合产品配制的小鼠-IgG。方法采用不同厂家的小鼠-IgG分别配制成不同的抗HCV-cAg酶结合物溶液,用酶联免疫法测定OD值。结果不同厂家的小鼠-IgG配制成的抗HCV-cAg酶结合物溶液,其检测结果有区别。结论配制抗HCV-cAg酶结合物溶液前应检测小鼠-IgG与产品的适配性。

Objective To optimize the formula of mouse-IgG.Methods The colorant A were prepared by various company,and OD values were determined by ELISA.Results The results of anti HCV-cAg solution with various companies were different.Conclusion It must be determined the suitability of mouse-IgG before using.