广西壳菜果遗传多样性的ISSR研究

Genetic Diversity of the Mytilaria laosensis in Guangxi Detected by ISSR Markers

利用ISSR分子标记技术,对广西壳菜果4个自然种群共43个个体进行了遗传多样性研究.结果表明,广西壳菜果天然种群总体水平的遗传多样性较高,各个种群的遗传多样性水平存在差异,其中广西凭祥种群的遗传多样性水平最高,广西那坡种群的最低.通过PopGen32分析表明:有82.43%的遗传变异存在于种群内,而17.57%的遗传变异存在于种群间,遗传分化系数(Gst)为0.175 8,种群间的基因流(Nm)为2.344 7,种群间的遗传分化程度较低,基因流动足以抵制遗传漂变的影响.利用UPGMA法对4个种群进行聚类分析显示:广西凭祥、广西德保和广西靖西3个种群聚为一类,广西那坡种群单独聚为一类.

The genetic diversity of 43 individuals of Mytilaria laosensis populations in Guangxi province was studied using inter-simple sequence repeat(ISSR) markers.The results show that the general level of Mytilaria laosensis natural population genetic diversity in Guangxi is high.The genetic diversity level of four populations is different largely.The level of genetic diversity of Pingxiang population in Guangxi is the highest among the four populations,and that of Napo population in Guangxi is the lowest.The analysis of genetic differentiation shows that the 82.43% of genetic variation is in population,and the others is not in population by PopGen 32.Coefficient of genetic differentiation(Gst) among four populations is 0.175 8 and the gene flow(Nm) among Mytilaria laosensis is 2.344 7.It shows that the level of genetic differentiation is low and gene flow is sufficient to resist the effects of genetic drift.UPGMA cluster analysis shows that four populations can be classified two kinds,and the population of Pingxiang,Debao and Jingxi in Guangxi are divided into one kind and the population of Napo in Guangxi is divided into one kind alone.