云南小耳猪胰岛细胞肝内移植对糖尿病猴的治疗作用

The Treatment Effect of Intrahepatic Islets Xenotransplantation of Yunnan Miniature Pig to Diabetes Rhesus Monkey

目的探讨云南小耳猪胰岛细胞经门静脉肝内异种移植的可行性,观察其对恒河猴糖尿病的治疗作用.方法 STZ诱导法复制恒河猴糖尿病模型,经胃网膜右静脉输注分离纯化的云南小耳猪胰岛细胞,移植在糖尿病猴肝内,移植后使用小剂量无激素免疫抑制方案,监测移植前后胰岛素用量、血糖以及血清C肽水平和肝肾功能的变化,评价猪-猴异种胰岛细胞移植对恒河猴糖尿病的治疗作用.结果 STZ能成功诱导恒河猴糖尿病模型;移植后第3天实验组糖化血红蛋白较术前降低,而对照组糖化血红蛋白一直呈上升状态;实验组外源性胰岛素用量较术前明显减少(P<0.05),对照组胰岛素用量无明显变化;实验组C肽水平较术前升高(P<0.05),对照组C肽一直维持在较低水平;无栓塞、感染、出血,肝肾功能无明显异常改变.结论云南小耳猪-恒河猴异种胰岛细胞移植能够降低糖尿病猴的高血糖状态,改善糖尿病症状,无明显副作用.

Objective To explore the treatment effect of intrahepatic islets xenotransplantation of yunnan miniature pig to diabetes Rhesus monkey.Methods Rhesus monkeys were induced by streptozotocin(STZ),then were perfused with purified porcine islets through the right gastroepiploic vein.Small dose immunosuppressive regimen without hormone was used.The dose of insulin,glycated hemoglobin,C peptide,blood glucose lever,and function of livers and kidneys of Rhesus monkeys were measured before and after transplantation.Results Type 1 diabetic Rhesus monkeys were successfully induced by STZ.The glycosylated hemoglobin in experimental group was decreased on the third day after transplantation,while the glycosylated hemoglobin in control group showed a rising state.The dose of insulin in experimental group was decreased significantly compared with pretransplantation(P<0.05),while the dose of insulin in control group had no obvious change.The serum C peptide levels in experimental group were elevated compared with preoperative levels(P<0.05),but the serum C peptide in control group was maintained at a low level.Embolism,infection and hemorrhage was not found.The function of livers and kidneys was maintained normal.Conclusion The intrahepatic islets xenoransplantation through the right gastroepiploic vein can decrease the high plasma glucose level of diabetic Rhesus monkeys,improve the symptoms of diabetes,and not lead to adverse reaction.