Survivin基因和EphB4基因在金黄地鼠颊囊癌变过程中的mRNA表达及意义

The Transcriptional Expression of Survivin and EphB4 in Hamster Buccal Pouch Carcinogenesis.

目的:探讨survivin基因和EphB4基因表达在金黄地鼠颊囊癌变过程中的作用。方法:采用DMBA诱导金黄地鼠颊囊动态癌变的动物模型,应用逆转录聚合酶链反应(RT-PCR)技术检测survivin和EphB4在各组组织中的表达。结果:Survivin mRNA在正常组、上皮单纯增生组、上皮异常增生组和鳞癌组的阳性表达率依次增加,除正常组与上皮单纯增生组无显著性差异外(P>0.05),其余任两组均有显著性差异(P<0.05)。EphB4 mRNA在以上任两组中的阳性表达率无显著性差异(P>0.05)。结论:在转录水平,survivin的表达与口腔黏膜从正常到癌变的发生发展过程有关,而EphB4则与之无关。

Objective： To explore the transcriptional expression of survivin and EphB4 in malignant transformation of hamster buccal mucosa. Methods： Reverse transcriptase polymerase chain reaction （RT- PCR） technique was used to detect the expression of survivin and EphB4 in the hamster buccal pouch carcinogenesis model induced by DMBA. Results： The expression of survivin increased gradiently from normal oral mucosa to oral squamous cell carcinoma（OSCC） , there was no significant difference in survivin expression between normal epithelia and hyperplasia epithelia（ P 〉 0.05 ） , but a statistically significant difference was found between any two groups of the rest （ P 〈 0.05 ）. There were no significant differences in EphB4 expression between any two groups （P 〉 0.05）. Conclusion： At the transcriptional level,it is survivin but EphB4 that has been involved in the carcinogenesis of oral mucosa.