低剂量环磷酰胺联合树突状细胞瘤苗对荷舌鳞癌裸鼠的治疗观察

Treatment of Established Tongue Squamous Cell Carcinoma-bearing Nude Mice By Dendrtic Cells Vaccine Combined With Low-dose Cyclophosphamide.

目的:观察低剂量环磷酰胺与树突状细胞瘤苗联合应用对裸鼠体内人舌癌细胞的抑瘤作用。方法:培养人外周血单核细胞来源的树突状细胞(DC),冻融法制备Tca8113舌癌细胞可溶性抗原并体外致敏DC,将同源人T淋巴细胞与之共同培养诱导出抗原特异性的CTL,联合低剂量环磷酰胺(Cy)共同接种至荷人舌癌的裸鼠。结果:抗原致敏的DC瘤苗与小剂量环磷酰胺联合后能比单用DC瘤苗更有效的治疗荷舌癌的裸鼠,与对照组相比,肿瘤生长明显抑制,生存率有显著提高(P<0.05)。结论:以冻融抗原致敏的DC激活产生肿瘤特异性的CTL联合小剂量Cy能更有效的促进荷瘤宿主的免疫反应,具有显著的体内抑制舌癌的效果。

Objective： To investigate the antitumor effects of dendritic cells （DC） pulsed with lysates of Tca8113 cells combined with low- dose cyclophosphamide（Cy）. Methods： DC was cultured from peripheral blood monocytes（PBMC）. After pulsed in vitro with Tca8113 cells lysates, DC was incubated with T lymphocytes to generate antigen -specific CTL, the tumor - bearing nude mice were treated with CTL and low - dose Cy. Results： The combination treatment could inhibit the growth of tumor, and the survival time of tumor - bearing mice was markedly prolonged. Conclusion： Tumor antigen pulsed DC combined with low -dose Cy could induce effectively in vivo antitumor immune response.