摘要
目的:观察兔眼视网膜不同缺血时间再灌注后ERG的变化。方法:20只大白兔,双眼均行ERG测定后随机分成A组10只,均左眼视神经结扎30min,右眼暴露视神经后不结扎。B组10只,均左眼视神经结扎60min,右眼视神经结扎90min。分别测定再灌注0,3,24,48,168h ERG a、b波振幅(aA、bA)值。结果:未结扎组视网膜缺血—再灌注0,3,24,48,168h ERG aA、bA值未见显著性差异。结扎后兔ERG aA、bA值均明显下降,结扎30min与90min组ERG aA、bA值随着再灌注时间的延长逐渐上升,再灌注168h,结扎30min组的ERG aA、bA值恢复正常。结扎60min组,ERG aA、bA值在再灌注24h进一步下降,后又逐渐上升。结论:缺血60min,再灌注24h时视网膜功能受到进一步损伤,缺血30min和90min,再灌注期间未观察到视网膜功能进一步损伤的现象。
AIM： To observe the ERG changes during reperfusion after different retinal ischemia times in rabbits. METHODS： Twenty healthy rabbits were randomly divided into two groups （A, B） after electroretinography of both eyes. The retinal ischemia in the left eyes of the 10 rabbits in Group A was induced by temporary ligation of the optic nerve for 30min, and a sham procedure was performed on the right eyes. The retinal ischemia in the left and the right eyes of the 10 rabbits in Group B was induced by temporary ligation of the optic nerve for 60 and 90 min respectively. The amplitudes of a, b-wave of （aA, bA） ERG were recorded at 0, 3, 24, 48, 168h of reperfusion. RESULTS： No significant difference was found in aA, bA of ERG at 0, 3, 24, 48, 168h of reperfusion in rabbits served as non-ischemic controls. Both aA and bA decreased significantly after the ligation. The aA and bA gradually increased following the time of reperfusion in 30-min ischemia and 90-min ischemia group. The aA and bA of ERG in the 30-min ischemia group were recovered to be normal at 168h of reperfusion. The aA and bA of ERG in the 60-min ischemia group further decreased at 24h of reperfusion, and then gradually increased in the following period. CONCLUSIONS： The retinal function aggravation was found at 24h of reperfusion after 60-min ischemia. No reperfusion injury was found in 30-min or 90-min ischemia group.
出处
《国际眼科杂志》
CAS
2005年第4期659-662,共4页
International Eye Science
