摘要
目的探讨5-氨基乙酰丙酸(5-ALA)诱导原卟啉IX(PpIX)在大肠肿瘤内积聚的特性。方法腹腔注射DMH溶液(1,2-二甲肼)建立大肠癌大鼠模型。尾静脉注射5-ALA溶液,对正常、早癌及进展期癌3组大肠组织分别取样,用化学抽提法提取组织原卟啉IX并定量检测其含量。结果在正常组、早癌组和进展期癌组中,大肠组织原卟啉IX含量差别均具有统计学意义,其原卟啉IX含量的平均值分别为(622.966±61.85)ng/g、(763.098±83.93) ng/g、(844.164±87.56)ng/g。结论5-ALA诱导原卟啉IX在大鼠大肠正常组织和癌组织中的积聚差异显著,为大肠早癌的荧光光谱诊断提供了实验依据。
Objective To study characteristics of different intracellular accumulation of protoporphyrin Ⅸ （PpⅨ） induced by 5-aminolaevalinic acid（5-ALA） in normal and cancer colon tissue. Methods To set up SD rat model of colon cancer by weekly intraperitoneal injections of 1,2-Syn-Dimethylhydrazine dihydrochloride （DMH） liquor. Tail intravenous injections of 5-ALA at a dose of 25mg/kg body weight after 150min,chemical extraction assays of the PpⅨ content by fluorescence spectrophotometer were carried out. Results A significant difference of PpⅨ content was found in different tissue, with a mean of （622.966 ± 61.85）ng/g, （763.098 ± 83.93） ng/g and （844. 164 ± 87.56） ng/g, respectively corresponding to normal tissue, early cancer and advanced cancer tissue in rats colon. Conclusion 5-ALA can induce effective differences of PpⅨ content in normal and cancer colonic tissue and provide experimental gist for early photie dynamic diagnosis of colon cancer.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第4期308-310,313,共4页
Journal of Harbin Medical University
基金
国家"十五"重点科技攻关项目(2001BA706B-15)
