高效液相色谱法测定赛特铂胶囊含量

HPLC Determination of Content Capsules Satraplatin

目的:建立赛特铂胶囊含量测定的高效液相色谱法。方法:色谱柱为北京迪马 Diamonsil^(TM)C_(18)(250mm×4.6mm,5μm)柱,流动相为甲醇-水-2%醋酸(50:35:15),检测波长260 nm。结果:在浓度范围6～200 mg·L^(-1)内,赛特铂峰面积与浓度呈良好线性关系(r=0.9999),样品加样回收率(n=5)为98.2%,RSD为1.1%。结论:方法简便可靠,能够满足赛特铂胶囊的含量测定要求。

Objective： To develop an RP - HPLC for determination of satraplatin in capsules. Methods： The chromatographic conditions were ： column, Diamonsil^TM C18 （ 250 mm × 4. 6 mm, 5 μm ） ; mobile phase, methanol - water - 2% acetic acid （50 ： 35 ： 15 ） ; detection wavelength, 260 nm. Results： The calibration curves was linear over the concentration range of 6 - 200 mg· L^-1 and the average recovery（ n = 5 ） of satraplatin was 98.2%, RSD = 1.1%. Conclusion：This method is simple and accurate.