RP-HPLC法测定复方氨林巴比妥注射液三组分的含量

Determination of Three Component in Compound Anlin Babituo Injection

目的:建立 RP-HPLC 法测定复方氨林巴比妥注射液中三组分的含量。方法:采用 C_(18)色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.0025 mol·L^(-1)庚烷磺酸钠-三乙胺(35:65:0.05,用50%醋酸调节 pH 至8.0,流速为0.6 mL·min^(-1),检测波长220 nm,柱温30℃。结果:氨基比林、安替比林、巴比妥分离度好,且均有良好的线性关系,r 分别为0.9998,0.9998,0.9997。加样回收率(n=5)分别为100.0%(RSD=0.8%),100.7%(RSD=0.8%),100.6%(RSD=1.2%)。结论:该法专属性强,分离度好,准确简便,为复方氨林巴比妥注射液的含量测定提供实用可靠的方法。

Objective：To establish a method for quantification of three components in compound Anlin Babituo injection by RP-HPLC. Methods： RP-HPLC chromatography was performed with a C18 column（250 mm × 4. 6 mm,5μm）, acetonitrile - 0. 0025 mol · L^-1 sodium 1 - heptanesulfonate - triethylamine （ 35 ： 65 ： 0. 05, adjusted to pH 8.0 with 50% acetic acid）as a mobile phase,flow rate at 0. 6 mL · min^-1 ,UV detection at 220 nm,and the column temperature at 30℃. Results：Aminopyrine,antipyrine and barbital were separated better and showed calibrate linear plots. The mean recovery rates （ n = 5 ） were 100. 0% （ RSD = 0. 8% ）, 100. 7% （ RSD = 0. 8% ）, 100. 6% （RSD = 1.2% ）respectively. Conclusion： The chromatographic procedure offered a good selective, separate, accurate quantification of compound Anlin Babituo injection.