摘要
蛋白激酶MEK1/2的专一抑制剂PD98059可抑制茉莉酸甲酯(MeJA)诱导的拟南芥保卫细胞中H2O2的产生和气孔的关闭。MeJA和H2O2诱导气孔关闭后,再用PD98059处理,可使关闭的气孔重新开放,同样,外源PD98059处理,能使MeJA诱导增强的H2O2探针的荧光强度降低。此结果表明,类属于MAPKK的蛋白激酶MEK1/2参与了MeJA诱导的拟南芥气孔关闭的信号转导过程,其作用机制可能是通过调节MeJA诱导保卫细胞产生和积累H2O2而起作用。
The specific inhibitor PD98059 of MEK1/2 could inhibit the H2O2 production and stomatal closure induced by methyl jasmonate （MeJA） in guard cells of Arabidopsis thaliana. After stomatal closure induced by MeJA and H2O2, PD98059 treatment could induce reopening of close stomata, in the meantime, the fluorescence intensity of H2O2 probe dichlorofluorescein （DCF） decreased. These results indicated that the MEK1/2 involved in the mitogen-activated protein kinase （MAPK） signal transduction system of stomatal closure induced by MeJA and mediated the H2O2 generation in guard cells of Arabidopsis thaliana.
出处
《植物生理学通讯》
CSCD
北大核心
2005年第4期439-443,共5页
Plant Physiology Communications
基金
国家自然科学基金(303707765)
"973"项目(200CB114305)。
