hTR反义PS-ODN联合顺铂对人胃癌裸鼠皮下移植瘤作用的体内研究

The effect of Htr- antisense oligodeoxynucleotide in combination with DDP on human gastric carcinoma transplanted subcutaneously in nude mice in vivo

目的探讨hTR反义寡脱氧核苷酸(ASODN)联合顺铂对人胃癌裸鼠皮下移植瘤的治疗作用。方法30只裸鼠建立胃癌皮下移植瘤模型后,随机分成5组,予以不同条件处理,对照组(瘤周注射RPMI1640培养液)、ASODN组(瘤周注射反义寡脱氧核苷酸)、RODN组(瘤周注射随机寡脱氧核苷酸)、DDP组(瘤周注射化疗药物顺铂)、ASODN+DDP组(瘤周注射反义寡脱氧核苷酸和顺铂)。治疗后定期观察肿瘤体积,计算肿瘤抑制率。采用TRAPPCR-ELISA法检测移植瘤的端粒酶活性。结果ASODN+DDP组、ASODN组、DDP组和RODN组的最高肿瘤抑制率分别为94.2%、84.3%,92.8%和26.9%。在端粒酶活性检测中,4个治疗组间均有统计学意义。结论hTR反义PS-ODN对人胃癌裸鼠皮下移植瘤生长及端粒酶活性有一定抑制作用,且可增强DDP的上述作用。

Objective To study whether hTR antisense PS-ODN has anticancer effect and the effect of increasing the susceptibility of tumor to DDP, we explore the effect of hTR-antisense oligodeoxynucleotide in combination with DDP on human gastric carcinoma transplanted subcutaneously in nude mice in vivo. Methods The model of human gastric carcinoma transplanted subcutaneously was established in thirty nude mice, then divided randomly into 5 groups （Control group, ASODN group, RODN group, DDP group and ASODN＋DDP group）. The weight of nude mice was measured, and the tumor growth inhibitory rate was calculated. The relative telomerase activity was quantitatively measured by TRAP PCR-ELISA methods. Results The maximum tumor inhibitory rate in ASODN＋DDP group, ASODN group, DDP group and RODN group was 94.2 %, 84.3 %, 92.8 % and 26.9 % respectively. There was significant difference in the relative telomerase activity among four treatment groups. Conclusions The results suggested that hTR antisense PS-ODN may act as a specific tumor growth inhibitor and telomerase activity inhibitor may he in sequence specific manner, and have the effect of increasing the susceptibility, of transplanted tumor to DDP.